Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus.

Wednesday, 16th of November 2016 Print

J Virol Methods. 2016 Nov 9;239:58-60. doi: 10.1016/j.jviromet.2016.11.003. [Epub ahead of print]

Comparison of monoplex and duplex RT-PCR assays for the detection of measles virus.

Binkhamis K1 Gillis H1 Lafreniere JD1 Hiebert J2 Mendoza L2 Pettipas J3 Severini A2 Hatchette TF1 LeBlanc JJ4.

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Abstract

Rapid and accurate detection of measles virus is important for case diagnosis and public health management. This study compared the performance of two monoplex RT-PCR reactions targeting the H and N genes to a duplex RT-PCR targeting both genes simultaneously. The duplex simplified processing without compromising assay orologi replica performance characteristic.

Copyright © 2016. Published by Elsevier B.V.

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