DRIED BLOOD SPOTS ON FILTER PAPER AS AN ALTERNATIVE SPECIMEN FOR MEASLES DIAGNOSTICS: DETECTION OF MEASLES IMMUNOGLOBULIN M ANTIBODY BY A COMMERCIAL ENZYME IMMUNOASSAY.

Monday, 18th of February 2013

[source]Journal of Infectious Diseases[|source]

Full text, with tables and figures is at

http://jid.oxfordjournals.org/content/189/Supplement_1/S177.long

Abstract

BACKGROUND: We compared the results of a serum-based measles immunoglobulin M (IgM) test with results of tests using paired reconstituted dried filter paper blood spot (DBS) samples to assess the feasibility of using DBS samples for measles diagnostic procedures.

METHODS: We collected 588 paired serum and DBS samples from 349 children aged 8 months through 12 years at Mulago Hospital in Kampala, Uganda; of these samples, 513 (87%) were collected from children with a clinical diagnosis of measles 0-33 days after rash, and 75(13%) were collected from children hospitalized for other reasons. Eluted DBS and serum samples were tested using a commercial measles IgM enzyme immunoassay. Detection of viral RNA was attempted on a subset of 20 DBS by reverse-transcriptase polymerase chain reaction.

RESULTS: Among the 513 sample pairs collected from children with measles, the concordances for samples collected during days 0-6 and >1 week after rash were 95.7% and 100%, respectively (P<.01). The relative sensitivity and specificity of the DBS-based assay during the first week were 98.7% and 88.9%, respectively, and the sensitivity and specificity >1 week after rash were 100% and 100%, respectively. Viral RNA was detected in 5 (26%) of 19 DBS samples tested. Among 75 sample pairs collected from children hospitalized for other reasons, concordance was 94.7%.

CONCLUSIONS: DBS samples are a feasible alternative sample for measles diagnostic procedures in high-incidence settings.